DNA

Part:BBa_K5471006:Design

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)


Kozak sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was excised from an already existing plasmid backbone, and was immediately ready for use


Source

This part is directly taken from "scAAV GFP", and specifically from positions 1053 to 1058 (from 5' end) scAAV GFP was a gift from K. Reed Clark (Addgene plasmid # 21893)

References

https://www.addgene.org/21893/

Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, Mendell JT. Cell. 2009 Jun 12. 137(6):1005-17. 10.1016/j.cell.2009.04.021 PubMed 19524505